Citation: | FENG Yaoze, LI Jize, YUAN Xunran, KONG Liqin, WANG Yijian, ZHU Ming. Particle count method mediated by polystyrene microspheres for umami detection[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2023, 39(18): 270-276. DOI: 10.11975/j.issn.1002-6819.202305187 |
Umami substances play an important role in enhancing food flavor and ensuring human health, and biological sensing technology is an emerging method for detecting umami substances. Therefore, in this study, a highly sensitive detection method for monosodium glutamate (MSG) was developed based on competitive reactions that were mediated by polystyrene (PS) microspheres and MSG-BSA functionalized complexes. After washing and centrifugation, the polystyrene microspheres were decorated with MSG-BSA and competed with MSG to bind with magnetic beads (MB) loaded with MSG receptor T1R1 in the tested sample. The coupled complex was removed by magnetic separation, and the remaining polystyrene microspheres was counted by 15 s to achieve the detection of monosodium glutamate concentration. UV spectroscopy and Zeta potential analyzer were used to characterize the surface modification of polystyrene microspheres and magnetic beads. The characteristic absorption peaks of 250 nm and 270 nm were observed for the naked polystyrene microspheres labeled with MSG-BSA, while the characteristic absorption peaks of 600 nm and 210 nm were observed for T1R1-labeled magnetic beads. It was found that the particle size of both polystyrene microspheres and magnetic beads changed significantly, indicating the successful modification of their surface. To obtain the optimal detection performance, the concentration of polystyrene microspheres, magnetic beads, and incubation time were optimized. The optimal conditions for the experiment were identified as polystyrene microspheres diameter of 3 μm, magnetic beads diameter of 1 μm, concentration ratio of polystyrene microspheres and magnetic beads at 1:3, and incubation time of 10 min. Particle counter is highly sensitive and accurate in detecting the polystyrene microspheres state, and particles at different states present significant differences in resistance. This detection simplified the procedure and greatly improved the detection sensitivity. The response equation of the method to the tested monosodium glutamate solution was satisfactory with a fitting coefficient of R2=0.993 and a limit of detection of 5.17 pg/mL. Compared with other methods, this method yielded better linear range and detection limit.To verify the specificity of the detection system for taste substance detection, four interfering substances, namely citric acid, sucrose, benzalkonium chloride, and sodium chloride, were selected. The response value of the interference signal was negligible compared to that of monosodium glutamate, indicating the high specificity of this detection system.Three sets of monosodium glutamate (MSG) solutions with the same concentration in parallel configurations were tested, with each set tested 10 times. The intra-group and inter-group relative standard deviation (RSD) was calculated, demonstrating that this method provided good stability for MSG detection. Moreover, the sensitivity and applicability of the developed detection method for actual sample analysis were verified using commercially available crucian carp, carp, and grass carp as examples. After a series of pre-processing operations, the response values of different concentrations of supernatant from crucian carp, carp, and grass carp were determined. The results showed that as the concentration of the supernatant increased and within the linear response range of the method, the response value of the particle counter also increased, indicating that this method can be used to perceive the concentration of umami substances in fish samples. This study demonstrated that the developed method had good accuracy and sensitivity, showing great application potential in food preservation and quality evaluation.
[1] |
KENZO K. Glutamate: From discovery as a food flavor to role as a basic taste ( umami)[J]. The American Journal of Clinical Nutrition, 2009, 90(3): 719-722. doi: 10.3945/ajcn.2009.27462D
|
[2] |
朱忆雯,张宁龙,姜水,等. 食品鲜味感知研究进展[J]. 中国食品学报,2021,21(1):1-16.
ZHU Yiwen, ZHANG Ninglong, JIANG Shui, et al. The research progress on food umami perception[J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21(1): 1-16. (in Chinese with English abstract)
|
[3] |
HELEN N. Update on food safety of monosodium l-glutamate(MSG)[J]. Pathophysiology, 2017, 24(4): 243-249. doi: 10.1016/j.pathophys.2017.08.001
|
[4] |
COULIER L, BAS R, HEKMAN M, et al. Comprehensive analysis of umami compounds by ion-pair liquid chromatography coupled to mass spectrometry[J]. Journal of Food Science, 2011, 76(7): 1081-1087. doi: 10.1111/j.1750-3841.2011.02306.x
|
[5] |
SHIGA K, YAMAMOTO S, NAKAJIMA A, et al. Metabolic profiling approach to explore compounds related to the umami intensity of soy sauce[J]. Journal of Agricultural and Food Chemistry, 2014, 62(29): 7317-7322. doi: 10.1021/jf501173r
|
[6] |
LIN H, YU X, FANG J, et al. Flavor compounds in pixian broad-bean paste: Non-volatile organic acids and amino acids[J]. Molecules, 2018, 23(6): 1299. doi: 10.3390/molecules23061299
|
[7] |
蒋雪松,王维琴,许林云,等. 农产品/食品中农药残留快速检测方法研究进展[J]. 农业工程学报,2016,32(20):267-274.
JIANG Xuesong, WANG Weiqin, XU Linyun, et al. Review on rapid detection of pesticide residues in agricultural and food products[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2016, 32(20): 267-274. (in Chinese with English abstract)
|
[8] |
蒋雪松,王剑平,应义斌,等. 用于食品安全检测的生物传感器的研究进展[J]. 农业工程学报,2007,23(5):272-277.
JIANG Xuesong, WANG Jianping, YING Yibin, et al. Recent advances in biosensors for food safety detection[J]. Transactions of the Chinese Society of Agricultural Engineering(Transactions of the CSAE), 2007, 23(5): 272-277. (in Chinese with English abstract)
|
[9] |
廖佳敏,杨华,孙鹏宇,等. 生物传感器发展研究综述[J]. 中国高新科技,2022(12):118-120.
LIAO Jiamin, YANG Hua, SUN Pengyu, et al. Review of biosensor development research[J]. China High and New Technology, 2022(12): 118-120. (in Chinese with English abstract)
|
[10] |
AHN S, AN J, SONG H, et al. Duplex bioelectronic tongue for sensing umami and sweet tastes based on human taste receptor nanovesicles[J]. ACS Nano, 2016, 10(8): 7287-7296. doi: 10.1021/acsnano.6b02547
|
[11] |
朱成喜,刘东,李玉叶,等. 比率电化学传感技术在农产品真菌毒素检测中的研究进展[J]. 农业工程学报,2022,38(5):259-268.
ZHU Chengxi, LIU Dong, LI Yuye, et al. Research progress on ratiometric electrochemical sensors for the detection of mycotoxins in agricultural products[J]. Transactions of the Chinese Society of Agricultural Engineering(Transactions of the CSAE), 2022, 38(5): 259-268. (in Chinese with English abstract)
|
[12] |
王俊,胡桂仙,于勇,等. 电子鼻与电子舌在食品检测中的应用研究进展[J]. 农业工程学报,2004,20(2):292-295.
WANG Jun, HU Guixian, YU Yong, et al. Research and application of electronic nose and electronic tongue in food inspection[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2004, 20(2): 292-295. (in Chinese with English abstract)
|
[13] |
KONG L, WANG Y, SHU G, et al. Kinetics of a new porcine taste-bud tissue biosensor for the detection of umami substances and their synergistic effect[J]. Biosensors and Bioelectronics, 2022, 210: 114304. doi: 10.1016/j.bios.2022.114304
|
[14] |
WEI X, QIN C, GU C, et al. A novel bionic in vitro bioelectronic tongue based on cardiomyocytes and microelectrode array for bitter and umami detection[J]. Biosens Bioelectron, 2019, 145: 111673. doi: 10.1016/j.bios.2019.111673
|
[15] |
刘海. 基于T1R1-T1R3结构的鲜味识别的分子机制研究[D]. 上海:上海海洋大学,2019.
LIU Hai. Research on the Molecular Mechanism of Umami Recognition by T1R1-T1R3[D]. Shanghai: Shanghai Ocean University, 2019. (in Chinese with English abstract)
|
[16] |
LIU J, ZHANG N, LI J, et al. A novel umami electrochemical biosensor based on AuNPs @ZIF-8/Ti3C2 MXene immobilized T1R1-VFT[J]. Food Chemistry, 2022, 397: 133838. doi: 10.1016/j.foodchem.2022.133838
|
[17] |
RODRIGUEZ T, AJINE M, ORZAN A, et al. Label-free protein detection using a microfluidic Coulter-counter device[J]. Sensors and Actuators B:Chemical, 2014, 190: 922-927. doi: 10.1016/j.snb.2013.09.038
|
[18] |
DEBLOIS R, BEAN C. Counting and sizing of submicron particles by resistive pulse technique[J]. Review of Scientific Instruments, 1970, 41(7): 909-1000. doi: 10.1063/1.1684724
|
[19] |
WANG Z, LIU J, YANG Y, et al. Versatile biosensing toolkit using an electronic particle counter[J]. Analytical Chemistry, 2021, 93(15): 6178-6187. doi: 10.1021/acs.analchem.1c00231
|
[20] |
ITO T, SUN L, CROOKS R. Simultaneous determination of the size and surface charge of individual nanoparticles using a carbon nanotube-based coulter counter[J]. Analytical Chemistry, 2003, 75(10): 2399-2406. doi: 10.1021/ac034072v
|
[21] |
HUA Z, YU T, LIU D, et al. Recent advances in gold nanoparticles-based biosensors for food safety detection[J]. Biosensors and Bioelectronics, 2021, 179: 113076. doi: 10.1016/j.bios.2021.113076
|
[22] |
HE H, NIE R, LU P, et al. Low-cost and convenient microchannel resistance biosensing platform by directly translating biorecognition into a current signal[J]. Analytical Chemistry, 2021, 93(45): 15049-15057. doi: 10.1021/acs.analchem.1c03006
|
[23] |
毛燕,黄小林,许恒毅,等. 免疫磁分离技术在食源性单增李斯特菌检测中应用的研究进展[J]. 食品工业科技,2015,36(8):351-355. doi: 10.13386/j.issn1002-0306.2015.08.065
MAO Yan, HUANG Xiaolin, XU Hengyi, et al. Research advance on immunomagnetic separation for detection of foodborne Listeria monocytogenes[J]. Science and Technology of Food Industry, 2015, 36(8): 351-355. (in Chinese with English abstract) doi: 10.13386/j.issn1002-0306.2015.08.065
|
[24] |
REN L, FENG W, HONG F, et al. One-step homogeneous micro-orifice resistance immunoassay for detection of chlorpyrifos in orange samples[J]. Food Chemistry, 2022, 386: 132712. doi: 10.1016/j.foodchem.2022.132712
|
[25] |
NIE R, HE H, LU P , et al. A homogeneous microchannel resistance sensor based on target-induced aggregation of polystyrene microspheres[J]. Sensors and Actuators B: Chemical, 2022, 369: 132277.
|
[26] |
陈翊平,何慧禹,聂荣彬,等. 一种基于绝缘微球浓度变化导致微通道电阻改变的生物传感检测方法. CN112415058B[P]. 2021-12-14.
|
[27] |
CHEN R, DONG Y. Z, HONG F, et al. Polydopamine nanoparticle-mediated, click chemistry triggered, microparticle-counting immunosensor for the sensitive detection of ochratoxin A[J]. Journal of Hazardous Materials, 2022, 428: 128206. doi: 10.1016/j.jhazmat.2021.128206
|
[28] |
郑胜彪,唐婧. 紫外分光光度法同时测定鸡精中谷氨酸钠和呈味核苷酸[J]. 中国调味品,2009,34(5):92-94.
ZHENG Shengbiao, TANG Jing. Simultaneous determinations of sodium glutamate and I+G in chicken essence by the ultraviolet spectrophotometric method[J]. China Condiment, 2009, 34(5): 92-94. (in Chinese with English abstract)
|
[29] |
邵宏宏,周秀锦,周向阳,等. 抑制型离子色谱法测定海产品中的有机酸类鲜味物质[J]. 中国调味品,2014,39(3):94-97.
SHAO Honghong, ZHOU Xiujin, ZHOU Xiangyang, et al. Simultaneous determination of umami substances of organic acids in marine products by suppressed ion chromatography[J]. China Condiment, 2014, 39(3): 94-97. (in Chinese with English abstract)
|
[30] |
LI J, WANG W, LIU J, et al. Human-like performance umami electrochemical biosensor by utilizing co-electrodeposition of ligand binding domain T1R1-VFT and Prussian blue[J]. Biosens Bioelectron, 2021, 193: 113627. doi: 10.1016/j.bios.2021.113627
|
[31] |
FACURE M, MERCANTE L, MATTOSO L, et al. Detection of trace levels of organophosphate pesticides using an electronic tongue based on graphene hybrid nanocomposites[J]. Talanta, 2017, 167: 59-66.
|
[32] |
AKHAVAN T, BINGER D, BLACKWOOD J, et al. A homogeneous chemiluminescent immunoassay method[J]. Journal of the American Chemical Society, 2013, 135(11): 4191-4194. doi: 10.1021/ja312039k
|
[33] |
TIAN Y, WANG P, DU L, et al. Advances in gustatory biomimetic biosensing technologies: In vitro and in vivo bioelectronic tongue[J]. TrAC Trends in Analytical Chemistry, 2022, 157: 116778. doi: 10.1016/j.trac.2022.116778
|
[34] |
WANG Y, KONG L, SHU G, et al. Development of sensitive and stable electrochemical impedimetric biosensor based on T1R1 receptor and its application to detection of umami substances[J]. Food Chemistry, 2023 , 423: 136233.
|
[1] | Zhang Xin, Yang Jianhua, Wang Weizhou, Jing Tianjun, Zhang Man. Construction and application of evaluation indexes for ruralmicro-energy-grid[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2020, 36(6): 196-205. DOI: 10.11975/j.issn.1002-6819.2020.06.023 |
[2] | Li Ming, Zhou Changji, Ding Xiaoming, Wei Xiaoming, Huang Shangyong, He Yanping. Heat insulation and storage performances of polystyrene-brick composite wall in Chinese solar greenhouse[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2016, 32(1): 200-205. DOI: 10.11975/j.issn.1002-6819.2016.01.028 |
[3] | Yuan Xiaoqing, Kong Qingxin, Li Qifeng, Li Lin, Li Daoliang. Evaluation method for application of internet of things for aquaculture[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2015, 31(4): 258-265. DOI: 10.3969/j.issn.1002-6819.2015.04.036 |
[4] | Qi Fei, Zhou Xinqun, Bao Shunshu, Ding Xiaoming, Wei Xiaoming, Lian Qinglong. Expression and evaluation method of integrated modes for protected horticulture engineering[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2013, 29(8): 195-202. |
[5] | Wang Defu. Experimental study of particle-evaluation equipment for roughage[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2007, 23(2): 128-131. |
[6] | Cao Shun′ai, Wu Cifang, Yu Wanjun. Method for environmental evaluation of land abroad and its application at Hangzhou City[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2006, 22(10): 263-266. |
[7] | Wang Ailing, Zhao Gengxing, Li Zhanjun. Integrated evaluation method for project post-evaluation of land consolidation benefits[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2006, 22(4): 58-61. |
[8] | Fan Jinmei, Wang Lei, Xue Yongsen. Evaluation of the benefit of land consolidation and rehabilitation[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2005, 21(13): 116-118. |
[9] | Kong Xiaoling, Jiang Deyun, Wei Shan, Yang Haidong. Comparative study on muscle tenderness evaluating methods[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 2003, 19(4): 216-219. |
[10] | ZHAO Geng-xing, LI Yu-huan, Li Qiang. Quantitative and Automatic Evaluation Methods Supported by GIS of Agricultural Land[J]. Transactions of the Chinese Society of Agricultural Engineering (Transactions of the CSAE), 1999, 15(3): 219-223. |