Primary study on technological conditions for chitosanase-producing microorganism

Chitosan is a high polymer and a cation exists only in nature, which can be biologically degraded and applied in the field of agriculture, medicine and food, etc. Low weight molecular of chitosan has better solubility and physiological functions than chitosan. The enzymic hydrolysis has some advantages of easy-controllable reaction, high security of products and less environmental pollution. Thus, the method and conditions for enzymic hydrolysis of chitosn are important. This paper deals with the incubation conditions for chitosanase producing microorganism basically and DNS method for measuring enzyme activity of chitosanase. The results showed that the maximum absorption wavelength of DNS method was 495 nm. The optimum incubation conditions for producing chitosanase were 60 h of the incubation time, 4.5 of the initial pH value and 50 mL of medium volume.