Preparation of wheat germ protein by combining alkali dissolution-acid deposition with α-amylase hydrolysis

For improving extraction effect of wheat germ protein, a new method which combines the dissolution with alkali and hydrolysis with α-amylase and deposition with acid was established. Compared with the traditional method of dissolution with alkali and deposition with acid, it can evidently increase the purity of wheat germ protein and yield ratio which were 94.5% and 81.36%, respectively. The purity of wheat germ protein which was obtained by this method was qualitatively determined by high performance capillary electrophoresis, and the results further prove that the purity of wheat germ protein is relatively higher. The molecular mass was determined by SDS-PAGE electrophoresis, and the results indicated that the molecular mass of wheat germ protein consisted of 35 kDa and 55 kDa.