Isolation of tea polysaccharides by D315 resin

The technology for isolation and purification of tea polysaccharides with D315 resin was studied. It was indicated that D315 resin was suitable for the preliminary isolation and purification of tea polysaccharides. Under the conditions of sample solution with pH 6.0～7.0, temperature 30℃ and 2.5 mg/mL of uronic acids, one polysaccharide NTPS which was mainly neutral sugar was obtained by collecting the solution flowing out and the fraction with water elution. The total sugar content of NTPS was 82.7% and its uronic acid content was 7.9%. Another polysaccharide ATPS which was mainly acid polysaccharides was obtained by collecting the fraction with 0.5 mol/L NaCl elution. The total sugar content of ATPS was 85.5% and its uronic acid content was 35.2%.