Optimization of purifying technology of tartary buckwheat total flavonoids with macroporous resin

In order to study the purifying technology of tartary buckwheat total flavonoids with DM-2 macroporous resin, the adsorption rates, desorption rates of macroporous resin were selected as monitoring parameters for separating efficiency. Response surface methodology(RSM) was employed in the study and a mathematical regression model for purification of tartary buckwheat total flavonoids with the macroporous resin was built. The technological parameters of the purifying process of tartary buckwheat total flavonoids with the macroporous resin were optimized. Results show that the optimal absorption parameters are adsorption velocity 2.0 mL/min, pH 2, solution concentration 2 mg/mL and the optimal desorbtion parameters are 71.05% ethanol as eluting solvent, pH 8.76, the desorption velocity 1 mL/min. This method is simple and feasible with good effect of purification, which can meet industrial requirements.