Optimization of extraction of polysaccharides from Hypsizigus marmoreus fruit-body

In order to optimize the technology for the extraction of polysaccharides from Hypsizigus marmoreus fruit-body, water was taken as extraction solvent and the effects of extraction temperature, extraction time, ratio of liquid to solid, ratio of ethanol to extract and extraction times on the yield of polysaccharides were investigated by the single factor experiments. Technology parameters were optimized by quadric regression orthogonal rotary design experiments. The results showed that the degree of the factors significantly influencing the yield of polysaccharides is in order of extraction temperature, extraction time, ratio of liquid to solid. The optimum extraction conditions are as follows: volume of ethanol to extract for precipitation is 2 times, extraction times 1, extraction temperature 95℃, extraction time 2.84 h, ratio of liquid to solid 11.6∶1. The yield of polysaccharides can be up to 4.47% under the optimum extraction conditions.