Separation of polyphenols from apple pomace based on different polarities and their antioxidative activities in vitro

To optimize the extraction technology of apple polyphenols and obtain higher content of powerful polyphenols from the extracts, polyphenols was extracted from the apple pomace by the method of liquid-liquid extraction and separated in the extraction solvent with different polarity. Antioxidative activities of the polyphenols for different polarity were measured in vitro by the method of 1,1-diphenyl-2-picrylhydrazyl radical and ferric reducing/antioxidant power. The results show that liquid-liquid extraction was a successful method for separation of the polyphenols for different polarity, and that their antioxidative activities in vitro order by polyphenols extacted with water > those extracted with n-butanol > extracted with ethyl acetate. The antioxidative activities of the polyphenols in vitro are positively correlated with their polarity. It can be concluded that powerful polyphenols may be better to obtain with the higher popularities extraction solvent when the polyphenols were extracted from apple pomace in industrial.