Optimization of technology for extracting antibacterial substances from pomegranate peel

In order to investigate the technology for extracting antibacterial substances from pomegranate peel, Staphylococcus aureus was used as indicator bacteria and diameter of inhibition zone was used as the activity monitoring parameter. The optimum extraction conditions were obtained by quadric regression orthogonal rotational experiments. The results show that the order of the main factors influencing the extracting effect from more important to less are as follows: ethanol concentration, extraction temperature, ratio of liquid to solid and extraction time; the optimum extraction conditions are that the concentration of ethanol is 60%, the ratio of liquid to solid is 10︰1, the temperature of extraction is 60℃ and the time of extraction is 4 h. Under the above mentioned conditions, the diameter of inhibition zone is 16.30 mm.