Online monitoring system for chlorophyll fluorescence parameters using LED excitation

A new in-situ monitoring system for chlorophyll fluorescence is proposed, using light-emitting diode (LED) excitation by means of the Pulse-amplitude-modulation (PAM) fluorescence detection technology. The system takes programmable power supply as drive power of LED array illumination with high sensitivity and signal-to-noise ratio. The system uses MINIPAM to measure fluorescence parameters such as Fs and Fm'. With the detection of temperature and PAR, and the measurement of other environmental factors, comprehensive analysis of the relationship between environmental factors and the chlorophyll fluorescence parameters such as the impact was conducted. The upper PC can control the power supply and process the data received from MINIPAM by software which programmed in Delphi7. The system takes the monitoring software which was programmed by Delphi7.0 on plants under the fluorescent light to conduct real-time online monitoring of parameters. The results show that, comparing with traditional method, the system has a lot of advantages such as high accuracy and good reliability and convenience. It will be a practical measurement and control system for photosynthesis ability and have a wide application foreground.