Optimum conditions of producing collagen polypeptide chelated Ca using pig bone

In order to take full advantage of pig bones resources, using pig bones as raw material to produce collagen polypeptide chelated Ca, the optimal conditions were studied in the paper. First, bone meal was hydrolyzed through lactic bacteria fermenting, and taking the content of Ca in the ferment liquid as indicator, the optimum extraction conditions were determined as that amount of sucrose, temperature, rotate speed, adding amount of strain and ferment time were 7%, 35℃, 120 r/min, 12% and 18 h, respectively. Then the concentrated ferment liquid under previous optimized conditions was chelated with collagen polypeptide, through single factor experiments and response surface analysis, the optimal chelate conditions were confirmed as follow: pH value 7.9, temperature 40℃, ratio of concentrated liquid to collagen polypeptide liquid 7:1, time of chelate 4 h and adding multiple of alcohol 11. Under these conditions, the chelate radio reached to 79.9%. The research can provide a reference for further developing of pig bones.