Bioactive peptides produced from ultrasonic-pretreated swine blood by microwave-assisted hydrolyzation

For making full use of livestock blood proteins and developing functional blooding biological materials, swine hemocytes were disintegrated by ultrasonic waves, and hemoglobins were hydrolyzed by Alcalase assisted by microwave. The results showed that the optimum parameters of ultrasonic disintegration were as follows: the volume ratio of water and hemocytes was 5:1, ultrasonic power 300 W, ultrasonic disintegration time 25 minutes. The 500 W microwave was used when hemocytes were hydrolyzed by Alcalase, and the optimum technology parameters were that the enzyme dose was 6.77% of volume ratio, enzymolysis temperature was 54℃ and enzymolysis time was 11 minutes, the degree of hydrolysis of hemoglobin was 23.63%, and the productions were almost oligopeptide with 635.7- 1 151.1 Da. Those could provide a quick extraction method of swine blood bioactive peptide for further study, and it had well value in practical application.