Supercritical CO2 fluid extraction technology and fatty acid composition of blackberry seed oil

In order to obtain high-quality healthy oil, supercritical CO2 fluid extraction was used to extract oil from blackberry seeds, and GC-MS was used to analyze the fatty acid composition of the oil. The optimum extraction parameters were confirmed as follows: material grind degree 60 mesh, extraction pressure 20 MPa, separation pressure 10 MPa, temperature 45℃, and extraction time 30 min. Under the above conditions, the yield of oil was (17.73±0.19)%. GC-MS analysis revealed that blackberry seed oil is rich in unsaturated fatty acid, especially linoleic acid (58.04%), oleic acid (11.76%) and linolenic acid (8.38%), accounting for 78.18% of the total fatty acid. The results can provide a reference for the comprehensive usage of black berry seeds.