Optimization of resin based technology for purification of polysaccharides from Ziziphus Jujuba cv. Huizao

Ziziphus Jujuba cv. Xinzheng Huizao is rich in kinds of nutrients, especially polysaccharides, which possess many biological effects on human bodies. However, the purification of polysaccharides from jujube after a primarily extraction step is still a difficult technology. In present research, enzyme combined with ultrasonic wave extraction, ethanol precipitation and ultrafiltration membrane filtration were employed to extract and preliminarily purify the polysaccharides from Ziziphus Jujuba cv. Xinzheng Huizao. The obtained raw polysaccharides were employed for further purification. Through the investigation of static adsorption and desorption of resins, the most suitable macroporous resin for the purification of jujube polysaccharides was selected and its dynamic adsorption and desorption behavior was studied. Furthermore, response surface experiments were conducted to investigate the effects of multifactor on the adsorption properties of macroporous resin for purification of jujube polysaccharide. The optimum adsorption conditions were 1.5 mL/min of in-flux rate, 2.2mg/mL of concentration of the feed liquid, 5.6 of pH value. Under this condition, the maximum dynamic adsorption rate of 19.52 mg/g could be obtained in theory. The optimized technical condition for dynamic desorption was obtained with 0.4 mol/L of NaCl, 60% of ethanol, 0.2 mol/L of HCl and 1.5 mL/min of flow rate. Under this condition, maximum dynamic desorption rate of 85.21% was achieved, and the purity of yield polysaccharides was 88.87%. Taken together, the present optimized technology is a highly effective method for the extraction and purification of polysaccharides from jujube, and has a great application and market potential in the medical and health care industries.