Purification technology of polyphenols from tea flower with macroporous absorbent resin

A process for isolating and purifying polyphenols from tea flower was developed. Proteins and other macromolecules in the polyphenols extracted by ethanol from tea flower were removed by ultrafiltration. Macroporous absorbent resin was chosen under the condition of static adsorption with ultrafiltrated samples, and finally the optimum tea flower polyphenol purification parameters were established with dynamic adsorption. Results indicate that HZ-806 resin has better adsorption and desorption properties than that of other four resins. The best adsorption conditions are found to be 4.0 mg/mL of polyphenol concentration, polyphenol sample volume is six times of the bed volume(BV), at pH 3.0 and a flow rate of 4 BV/h. Whereas the optimal desorption conditions are found to be a flow rate of 2 BV/h using 3.33 times BV of 70% ethanol for elution. The final adsorption rate and desorption rate are 97.5% and 98.20%, respectively. The purity of tea flower polyphenols increases from 19.43％ to 84.32%. That suggests that polyphenols can be obtained from tea flower effectively.